A Study of Polymerase Chain Reaction in Cerebrospinal Fluid for Diagnosis of Tuberculous Meningitis
نویسندگان
چکیده
Methods: All children having CNS illness and undergoing CSF examination were included in the study and were grouped into two. Group I (Study Group) were subjects with the clinical evidence along with one or more of the laboratory or radiological evidence for tuberculous meningitis. Group II (Control Group) comprised of subjects who were diagnosed to have CNS illness other than tuberculous meningitis undergoing CSF examination. CSF samples from all the subjects were subjected to AFB staining, culture and PCR for Mycobacterium tuberculosis.
منابع مشابه
EVALUATION OF DIRECT MICROSCOPY, CULTURE, AND POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF TUBERCULOUS MENINGITIS
In view of the importance of early diagnosis of tuberculous meningitis (TBM), the efficiency of the polymerase chain reaction (PCR), one of the most reliable and sensitive DNA-based assays, was compared with conventional methods (acid-fast microscopy and culture) for the detection of M. tuberculosis in cerebrospinal fluid (CSF) specimens from patients suspected of TBM. Of the 29 CSF specim...
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TBM is an endemic disease in developing countries [1]. At present 1/5th of the global annual new cases occur in India. Tuberculosis claims 100 lives per day, out of them 20% patients have TBM.Delay in diagnosis and so in the start of effective treatment results in poor prognosis and sequelae in up to 25% of cases [2]. Available methods of diagnosis of TBM were evaluated [3] and all of them were...
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